Skip to content

JASCO JASCO

  • News
  • Events
  • E-Store
  • My Account
  • Contact Us
  • Worldwide
Search
Click to view menu
MENUMENU
  • Products
    • Chromatography
      • HPLC
      • RHPLC
      • UHPLC
      • LC-MS
      • Preparative LC
      • Analytical SFC
      • Semi-Preparative SFC
      • Hybrid SFC
      • Fuel Analysis by SFC-FID
      • Preparative SFC
      • Supercritical Fluid Extraction
      • Chromatography Software
    • Molecular Spectroscopy
      • Circular Dichroism
      • High-Throughput CD
      • Vibrational CD
      • Circularly Polarized Luminescence (CPL)
      • Polarimeters
      • FTIR Spectrometers
      • FTIR Microscopy
      • FTIR Portable
      • Raman Microscopy
      • Palmtop Raman Spectrometer
      • Probe Raman
      • UV-Visible/NIR Spectrophotometers
      • UV-Visible/NIR Microscopy
      • Fluorescence Spectrophotometers
      • Film Thickness
      • Spectra Manager™ Suite
    • Refurbished
      • Refurbished HPLC Systems
      • HPLC Switching Valves
      • FTIR Accessories
  • Service
    • Service and Support Plans
    • Service Request Form
  • Applications
  • KnowledgeBase
  • Learning Center
    • Best Practice
      • Circular Dichroism Tips & Tricks for Biological Samples
      • CD Scale Calibration with ACS
      • Fluorescence Tips & Tricks
      • Raman Spectroscopy Tips & Tricks
    • Training Videos
      • ChromNAV
      • SF-NAV
      • Circular Dichroism
      • UV-Visible/NIR
      • Fluorescence
    • Training Seminars
      • Training Registration Form
    • Webinars
    • eBooks
    • Theory
      • Theory of Molecular Spectroscopy
      • Chromatography
  • About Us
    • President’s Message
    • Contact
    • History
    • Careers
  • News
  • Events
  • Worldwide
  • Shop
  • My Account
  • Contact Us

Home / Applications / High-Speed Analysis of Polycyclic Aromatic Hydrocarbons in Diesel Exhaust Particulate Matter using UHPLC

  • Industry

  • Technique

High-Speed Analysis of Polycyclic Aromatic Hydrocarbons in Diesel Exhaust Particulate Matter using UHPLC

By Jiawei Xu

PDF IconDownload This Application

January 5, 2024

Introduction

Polycyclic aromatic hydrocarbons (PAHs) are chemicals generated when fuels are incompletely combusted, which are contained in industrial and car exhaust and are known as the main source of air pollution. Some of them are categorized as carcinogens and these compounds are subjected to the regulations of governmental agencies such as EPA (Environmental Protection Agency) in U.S.A.

In this application, PAHs in diesel exhaust particulate matter are measured using Ultra High-performance Liquid Chromatography (UHPLC) with PDA detection.

JASCO X-LC system

Experimental

Keywords

230007X

Results

Figure 1 shows the chromatogram of the PAH standard mixture (EPA 610) and the contour plot. 16 compounds were clearly separated within 6 minutes.

Figure 1. Chromatogram of the PAH standard mixture. 1: Naphthalene, 2: Acenaphthylene, 3: Acenaphthene, 4: Fluorene, 5: Phenanthrene, 6: Anthracene, 7: Fluoranthene, 8: Pyrene, 9: Benzo[a]anthracene, 10: Chrysene, 11: Benzo[b]fluoranthene, 12: Benzo[k]fluoranthene, 13: Benzo[a]pyrene, 14: Dibenzo[ah]anthracene, 15: Benzo[ghi]perylene, 16: Indeno[123-cd]pyrene
Figure 2 shows the chromatogram and the contour plot of the sample solution that is extracted from diesel exhaust particulate matter with Soxhlet extraction. It was confirmed from the spectral information that PAHs were detected.

Figure 2. Chromatogram of extract from diesel exhaust particulate matter. The peak numbers and corresponding compounds are the same as in figure 2.

Sample preparation.

Sample solution was filtrated using 0.2 um membrane filter after Soxhlet extraction.

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.

Featured Products:

  • Single Quadrupole LC-MS

  • SFC-4000 Analytical SFC for routine separation to method development.

    Analytical SFC System

  • LC-4000 Series Preparative HPLC

About the Author

Jiawei Xu

JASCO Application Note

High-Speed Analysis of Polycyclic Aromatic Hydrocarbons in Diesel Exhaust Particulate Matter using UHPLC

Introduction

Polycyclic aromatic hydrocarbons (PAHs) are chemicals generated when fuels are incompletely combusted, which are contained in industrial and car exhaust and are known as the main source of air pollution. Some of them are categorized as carcinogens and these compounds are subjected to the regulations of governmental agencies such as EPA (Environmental Protection Agency) in U.S.A.

In this application, PAHs in diesel exhaust particulate matter are measured using Ultra High-performance Liquid Chromatography (UHPLC) with PDA detection.

JASCO X-LC system

Experimental

Results

Figure 1 shows the chromatogram of the PAH standard mixture (EPA 610) and the contour plot. 16 compounds were clearly separated within 6 minutes.

Figure 1. Chromatogram of the PAH standard mixture. 1: Naphthalene, 2: Acenaphthylene, 3: Acenaphthene, 4: Fluorene, 5: Phenanthrene, 6: Anthracene, 7: Fluoranthene, 8: Pyrene, 9: Benzo[a]anthracene, 10: Chrysene, 11: Benzo[b]fluoranthene, 12: Benzo[k]fluoranthene, 13: Benzo[a]pyrene, 14: Dibenzo[ah]anthracene, 15: Benzo[ghi]perylene, 16: Indeno[123-cd]pyrene
Figure 2 shows the chromatogram and the contour plot of the sample solution that is extracted from diesel exhaust particulate matter with Soxhlet extraction. It was confirmed from the spectral information that PAHs were detected.

Figure 2. Chromatogram of extract from diesel exhaust particulate matter. The peak numbers and corresponding compounds are the same as in figure 2.

Sample preparation.

Sample solution was filtrated using 0.2 um membrane filter after Soxhlet extraction.

Keywords

230007X

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.
28600 Mary’s Court, Easton, MD 21601 USA • (800) 333-5272 • Fax: (410) 822-7526 • jascoinc.com/applications

Close

Designed in Tokyo. TRUSTED globally.

View our support plans

Connect with JASCO

  • Facebook
  • Twitter
  • LinkedIn
  • JASCO Sales
  • 800-333-5272

Receive the latest promotions and special offers

  • This field is for validation purposes and should be left unchanged.
  • Careers
  • Press Kit
  • JASCO Privacy Policy
  • Sitemap
  • Environmental Policy

© , JASCO. All Rights Reserved.