Magnetic Circular Dichroism using a PMCD-586 Permanent Magnet

By Heather Haffner

Introduction

Permanent Magnetic Circular Dichroism

Magnetic circular dichroism passes linearly polarized light through a material in a magnetic field parallel to the direction of the magnetic field, and the polarization plane is rotated. The use of a magnetic field to induce optical activity in molecules is also known as the Faraday effect.

magnetic circular dichroism

Magnetic Optical Rotation Dispersion (MORD) and Magnetic Circular Dichroism (MCD) can be used to observe magnetic field-induced effects on optically active molecules. MCD is widely used today to probe the local environment of protein molecules. Chromophores with large magnetic moments arising from either rotational symmetries (aromatics, porphyrins), unpaired spins (metal complexes), or both (hemes) are sensitive to electronic perturbations and therefore provide information regarding the molecule’s electronic state. The MCD signal intensity is proportional to the magnetic field strength, which can be applied by using a permanent magnet.

Previously, in order to generate a strong magnetic field (greater than 1 Tesla) a large electromagnet was required. However, electromagnets are not easily located inside the sample compartment of a CD spectrometer due to its excessive weight (typically greater than 60 kg). The PMCD-586 permanent magnet has a magnetic field strength of 1.6 Tesla and easily fits inside the sample compartment. The direction of the magnetic field can be changed by simply reversing the direction of the magnet.

This application note provides examples of the J-1500 CD spectrometer and PMCD-586 permanent magnet to obtain MCD measurements.

Experimental

Measurement conditions

	Neodymium glass	Cytochrome C	Cobalt chloride (II) 6 Hydrate
Data Acquisition Interval	0.5 seconds	2 seconds	2 seconds
Spectral Bandwidth	1 nm	1 nm	1 nm
Scan Speed	50 nm/min/td>	20 nm/min	100 nm/min
Path Length		0.2 cm	0.5 cm

Keywords

180-CD-0008, J-1500, circular dichroism, magnetic circular dichroism, Faraday effect, UV-Vis, NIR, biochemistry, chemical

Results

Neodymium glass is optically inactive. However, when subjected to a magnetic field an MCD spectrum from the UVVisible to NIR region can be observed. The spectra in Figure 1 show sharp MCD signals caused by strong optical absorption. Figure 2 shows that small MCD signals caused by weak optical absorptions can be enhanced by the strong 1.6 T magnetic field. MCD spectra illustrate sharp peaks that cannot be separated in the absorption spectra.

Magnetic Circular Dichroism Spectrums

If the direction of the magnetic field is the same as that of the incoming light, the magnetic field can be defined as being in the forward direction. If the direction of the magnetic field is opposite to that of the incoming light, it is defined as the magnetic field in the reverse direction. This is depicted in Figure 3. By changing the direction of the magnetic field, the MCD spectra can also be reversed.

Magnetic field in the forward direction(left) and the magnetic field in the reverse direction(right)

MCD of Fe (III) cytochrome c

MCD is widely used for the structural analysis of hemoprotein samples and several studies of myoglobin¹, hemoglobin², cytochrome b₅3-4, cytochrome c3,5-6, cytochrome P-4507 , and horseradish peroxidase8 are reported. The MCD spectra of the Soret band of Fe(III) cytochrome in solution are shown in Figure 4. If the CD spectrum obtained in the absence of a magnetic field is subtracted from the MCD spectra, symmetric MCD spectra can be observed.

If the Circular Dichroism spectrum obtained in the absence of a magnetic field is subtracted from the MCD spectra, symmetric MCD spectra can be observed.

MCD of cobalt chloride (II) 6 hydrate

An aqueous solution of cobalt chloride (II) 6 hydrate exists as a hexacoordinated structure where the cobalt ion is coordinated with six water molecules. Conversely, cobalt chloride 6 hydrate in a concentrated HCl solution exists as a tetrahedron complex in which the cobalt ion is coordinated to four chloride ions. The MCD spectra illustrate drastic changes based on the change in the electronic state of the cobalt ion and are shown in Figures 5 and 6.

Conclusion

This application note illustrates that the J-1500 CD spectrometer and the PMCD-586 permanent magnet can be used to obtain magnetic circular dichroism spectra for a variety of samples.

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.

About the Author

Introduction

Permanent Magnetic Circular Dichroism

magnetic circular dichroism

This application note provides examples of the J-1500 CD spectrometer and PMCD-586 permanent magnet to obtain MCD measurements.