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Home / Applications / Separation of Basic Drugs by Supercritical Fluid Chromatography

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Separation of Basic Drugs by Supercritical Fluid Chromatography

By Heather Haffner

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January 8, 2024

Introduction

It is well known that SFC with a column of high polarity material shows the same retention action as normal phase chromatography, and therefore it is believed to be difficult to separate aqueous high polarity components. However, if a little volatile acid, base, or salt is added to modifier solvent(alcohol or etc.), the shape of polar component’s peak can be improved, and components with a long retention time can be eluted with an appropriate retention time.

Various basic drugs were separated using the 2-Ethylpyridine column with ammonium acetatein methanolas modifier solvent.

Experimental

Keywords

747011S

Results

Chromatogram and contour plot of standard mixture of basic drugs (220nm) are shown in Figure 1. As shown, polar components such as Berberine and Maleic were also eluted.

Figure1. Chromatogram of standard mixture of basic drugs
1: Caffeine, 2: Hexobarbital, 3: Amitriptyline, 4: Chlorpheniramine, 5: Imipramine, 6: Quinine, 7:Atropine, 8: Acetaminophen, 9: Berberine,10: Maleic acid
This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.

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About the Author

JASCO Application Note

Separation of Basic Drugs by Supercritical Fluid Chromatography

Introduction

It is well known that SFC with a column of high polarity material shows the same retention action as normal phase chromatography, and therefore it is believed to be difficult to separate aqueous high polarity components. However, if a little volatile acid, base, or salt is added to modifier solvent(alcohol or etc.), the shape of polar component’s peak can be improved, and components with a long retention time can be eluted with an appropriate retention time.

Various basic drugs were separated using the 2-Ethylpyridine column with ammonium acetatein methanolas modifier solvent.

Experimental

Results

Chromatogram and contour plot of standard mixture of basic drugs (220nm) are shown in Figure 1. As shown, polar components such as Berberine and Maleic were also eluted.

Figure1. Chromatogram of standard mixture of basic drugs
1: Caffeine, 2: Hexobarbital, 3: Amitriptyline, 4: Chlorpheniramine, 5: Imipramine, 6: Quinine, 7:Atropine, 8: Acetaminophen, 9: Berberine,10: Maleic acid

Keywords

747011S

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.
28600 Mary’s Court, Easton, MD 21601 USA • (800) 333-5272 • Fax: (410) 822-7526 • jascoinc.com/applications

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