# Separation of Basic Drugs by Supercritical Fluid Chromatography

## Introduction

It is well known that SFC with a column of high polarity material shows the same retention action as normal phase chromatography, and therefore it is believed to be difficult to separate aqueous high polarity components. However, if a little volatile acid, base, or salt is added to modifier solvent(alcohol or etc.), the shape of polar component’s peak can be improved, and components with a long retention time can be eluted with an appropriate retention time.

Various basic drugs were separated using the 2-Ethylpyridine column with ammonium acetatein methanolas modifier solvent.

## Result

Chromatogram and contour plot of standard mixture of basic drugs (220nm) are shown in Figure 1. As shown, polar components such as Berberine and Maleic were also eluted.