Supercritical Fluid Extraction of Bisphenol A in Epoxy Resin

By Kohei Tamura

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July 9, 2025

Introduction

Jasco Supercritical Fluid Extraction

Bisphenol A considered as endocrine disruptors were extracted using Supercritical Fluid Extraction (SFE) with carbon dioxide and analyzed by reversed-phase HPLC.

Fig.1 shows the chromatograms of the extracts from epoxy resins used for coating of artificial leather, electrical cables, etc.

Fig. 1 Chromatograms of bisphenol A-STD sample and epoxyresin extraction sample

Pretreatment

Sample
↓
Extraction by supercritical fluid
↓
Recovery of extracted solution
↓
Concentration
↓
Sample volume in measuring flask by
CH3OH/H2O (50/50)
↓
Injection into HPLC

Conditions

SFE

System:SCF-201
Extraction vessel:1 mL
CO2 flow rate:3.0 mL/min
CH3OH flow rate:0.3 mL/min
Extraction Pressure:25 Mpa
Sample:Synthetic leather
Covering for AC cable

HPLC

Column:CrestPak C18S (4.6 mm I.D. x 150 mm L.)
Eluent:CH3OH / H2O (50/50)
Wavelengths:Ex 282 nm, Em 306 nm, Gain x 100
Flow rate:1.0 mL/min
Sample:Standard (100 ppb)
Extracts of coating material of artificial
leather and electrical cable
Injection volume:10 µL

Keywords

Bisphenol A; STD, epoxy resins; SFE; Fluorescence detection; endocrine disruptor

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.

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About the Author

Mr. Tamura is a member of the applications team with a specialty in vibrational spectroscopy and particularly Raman and IR Imaging. He is also one of our experts in gas analysis by FTIR.