Introduction
Bisphenol A considered as endocrine disruptors were extracted using Supercritical Fluid Extraction (SFE) with carbon dioxide and analyzed by reversed-phase HPLC.
Fig.1 shows the chromatograms of the extracts from epoxy resins used for coating of artificial leather, electrical cables, etc.
Pretreatment
Sample
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Extraction by supercritical fluid
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Recovery of extracted solution
↓
Concentration
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Sample volume in measuring flask by
CH3OH/H2O (50/50)
↓
Injection into HPLC
Conditions
SFE
| System: | SCF-201 |
| Extraction vessel: | 1 mL |
| CO2 flow rate: | 3.0 mL/min |
| CH3OH flow rate: | 0.3 mL/min |
| Extraction Pressure: | 25 Mpa |
| Sample: | Synthetic leather Covering for AC cable |
HPLC
| Column: | CrestPak C18S (4.6 mm I.D. x 150 mm L.) |
| Eluent: | CH3OH / H2O (50/50) |
| Wavelengths: | Ex 282 nm, Em 306 nm, Gain x 100 |
| Flow rate: | 1.0 mL/min |
| Sample: | Standard (100 ppb) Extracts of coating material of artificial leather and electrical cable |
| Injection volume: | 10 µL |
Keywords
Bisphenol A; STD, epoxy resins; SFE; Fluorescence detection; endocrine disruptor