Supercritical Fluid Extraction of Bisphenol A in Epoxy Resin

By Kohei Tamura

Download PDF August 24, 2022

  • Industry

  • Technique

Introduction

Jasco Supercritical Fluid Extraction

Bisphenol A considered as endocrine disruptors were extracted using Supercritical Fluid Extraction (SFE) with carbon dioxide and analyzed by reversed-phase HPLC.

Fig.1 shows the chromatograms of the extracts from epoxy resins used for coating of artificial leather, electrical cables, etc.

Fig. 1 Chromatograms of bisphenol A-STD sample and epoxyresin extraction sample

Pretreatment

Sample
↓
Extraction by supercritical fluid
↓
Recovery of extracted solution
↓
Concentration
↓
Sample volume in measuring flask by
CH3OH/H2O (50/50)
↓
Injection into HPLC

Conditions

SFE

System:SCF-201
Extraction vessel:1 mL
CO2 flow rate:3.0 mL/min
CH3OH flow rate:0.3 mL/min
Extraction Pressure:25 Mpa
Sample:Synthetic leather
Covering for AC cable

HPLC

Column:CrestPak C18S (4.6 mm I.D. x 150 mm L.)
Eluent:CH3OH / H2O (50/50)
Wavelengths:Ex 282 nm, Em 306 nm, Gain x 100
Flow rate:1.0 mL/min
Sample:Standard (100 ppb)
Extracts of coating material of artificial
leather and electrical cable
Injection volume:10 µL

Keywords

Bisphenol A; STD, epoxy resins; SFE; Fluorescence detection; endocrine disruptor

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About the Author

Mr. Tamura is a member of the applications team with a specialty in vibrational spectroscopy and particularly Raman and IR Imaging. He is also one of our experts in gas analysis by FTIR.