Significant efforts to develop both laboratory and field-based detection assays for an array of potential biological threats started well before… Continue reading Rugged Single Domain Antibody Detection Elements for Bacillus anthracis Spores and Vegetative Cells
Leah Pandiscia, PhD
July 28, 2017
Regulation of protein activity is central to the complexity of life. The ability to regulate protein activity through exogenously added… Continue reading In Vitro Recombination of Non-Homologous Genes Can Result in Gene Fusions that Confer a Switching Phenotype to Cells
Leah Pandiscia, PhD
July 28, 2017
CaM-KIIN has evolved to inhibit stimulated and autonomous activity of the Ca2+/calmodulin (CaM)-dependent protein kinase II (CaMKII) efficiently, selectively, and… Continue reading Improving a Natural CaMKII Inhibitor by Random and Rational Design
Leah Pandiscia, PhD
July 28, 2017
Leah Pandiscia, PhD
July 28, 2017
Falcipain-2, a papain family cysteine protease of the malaria parasite Plasmodium falciparum, plays a key role in parasite hydrolysis of hemoglobin… Continue reading Regulatory Elements within the Prodomain of Falcipain-2, a Cysteine Protease of the Malaria Parasite Plasmodium falciparum
Leah Pandiscia, PhD
July 28, 2017
The exocyst is a large hetero-octomeric protein complex required for regulating the targeting and fusion of secretory vesicles to the… Continue reading Conservation of Helical Bundle Structure between the Exocyst Subunits
Leah Pandiscia, PhD
July 28, 2017
Leah Pandiscia, PhD
July 28, 2017
The process of antibody ontogeny typically improves affinity, on-rate, and thermostability, narrows polyspecificity, and rigidifies the combining site to the… Continue reading Ontogeny of Recognition Specificity and Functionality for the Broadly Neutralizing Anti-HIV Antibody 4E10
Leah Pandiscia, PhD
July 28, 2017
Mitochondrial transcription factor A (TFAM) had previously been shown to act as a damage associated molecular pattern with the ability… Continue reading Mechanism of Mitochondrial Transcription Factor A Attenuation of CpG-Induced Antibody Production
Leah Pandiscia, PhD
July 28, 2017
DksA controls transcription of genes associated with diverse stress responses, such as amino acid and carbon starvation, oxidative stress, and… Continue reading pH Dependence of the Stress Regulator DksA
Leah Pandiscia, PhD
July 28, 2017
Proteins that are post-translationally adducted with 2-(ω-carboxyethyl)pyrrole (CEP) have been proposed to play a pathogenic role in age-related macular degeneration,… Continue reading Lack of Involvement of CEP Adducts in TLR Activation and in Angiogenesis
Leah Pandiscia, PhD
July 28, 2017
There is ongoing interest to develop high affinity, thermal stable recognition elements to replace conventional antibodies in biothreat detection assays.… Continue reading Development and Evaluation of Single Domain Antibodies for Vaccinia and the L1 Antigen
Leah Pandiscia, PhD
July 28, 2017
Mutations in the ABCC6 ABC-transporter are causative of pseudoxanthoma elasticum (PXE). The loss of functional ABCC6 protein in the basolateral… Continue reading Regulation of ABCC6 Trafficking and Stability by a Conserved C-terminal PDZ-Like Sequence
Leah Pandiscia, PhD
July 28, 2017
The creation of a synthetic microbe that can harvest energy from sunlight to drive its metabolic processes is an attractive… Continue reading A Tale of Two Reductases: Extending the Bacteriochlorophyll Biosynthetic Pathway in E. coli
Leah Pandiscia, PhD
July 28, 2017
Single domain antibodies are the small recombinant variable domains derived from camelid heavy-chain-only antibodies. They are renowned for their stability,… Continue reading Evaluation of Disulfide Bond Position to Enhance the Thermal Stability of a Highly Stable Single Domain Antibody
Leah Pandiscia, PhD
July 28, 2017
Single domain antibodies (sdAbs) are the recombinantly-expressed variable domain from camelid (or shark) heavy chain only antibodies and provide rugged… Continue reading Contributions of the Complementarity Determining Regions to the Thermal Stability of a Single-Domain Antibody
Leah Pandiscia, PhD
July 28, 2017
Genetic leukoencephalopathies (gLEs) are a group of heterogeneous disorders with white matter abnormalities affecting the central nervous system (CNS). The… Continue reading A Founder Mutation in VPS11 Causes an Autosomal Recessive Leukoencephalopathy Linked to Autophagic Defects
Leah Pandiscia, PhD
July 28, 2017
The flexibility in the structure of calmodulin (CaM) allows its binding to over 300 target proteins in the cell. To… Continue reading The Effect of Macromolecular Crowding, Ionic Strength and Calcium Binding on Calmodulin Dynamics
Leah Pandiscia, PhD
July 28, 2017
A central event in Alzheimer’s disease is the accumulation of amyloid β (Aβ) peptides generated by the proteolytic cleavage of… Continue reading Amyloid Precursor Protein Translation Is Regulated by a 3’UTR Guanine Quadruplex
Leah Pandiscia, PhD
July 28, 2017
β-Lactamases are the major reason β-lactam resistance is seen in Gram-negative bacteria. To combat this resistance mechanism, β-lactamase inhibitors are… Continue reading Penam Sulfones and β-Lactamase Inhibition: SA2-13 and the Importance of the C2 Side Chain Length and Composition
Leah Pandiscia, PhD
July 28, 2017