Paleotemperatures inferred from the isotopic compositions (δ18O and δ30Si) of marine cherts suggest that Earth’s oceans cooled from 70 ±… Continue reading Reconstructed ancestral enzymes suggest long-term cooling of Earth’s photic zone since the Archean
Leah Pandiscia, PhD
July 28, 2017
Leah Pandiscia, PhD
July 28, 2017
Leah Pandiscia, PhD
July 28, 2017
The phosphorylation of agonist-occupied G-protein-coupled receptors (GPCRs) by GPCR kinases (GRKs) functions to turn off G-protein signaling and turn on… Continue reading Structural and Functional Analysis of a β2-Adrenergic Receptor Complex with GRK5
Leah Pandiscia, PhD
July 28, 2017
The glucocorticoid receptor (GR) is involved in the transcriptional regulation of genes associated with inflammation, glucose homeostasis, and bone turnover… Continue reading Agonist versus antagonist induce distinct thermodynamic modes of co-factor binding to the glucocorticoid receptor
Leah Pandiscia, PhD
July 28, 2017
Leah Pandiscia, PhD
July 28, 2017
Glucagon-like peptide (GLP-1) is an endogenous hormone that induces insulin secretion from pancreatic islets and modified forms are used to… Continue reading Helixconstraints and amino acid substitution in GLP-1 increase cAMP and insulin secretion but not beta-arrestin 2 signaling
Leah Pandiscia, PhD
July 28, 2017
The Escherichia coli protein RecA is responsible for catalysis of the strand transfer reaction used in DNA repair and recombination. Previous studies… Continue reading The effects of buffers and pH on the thermal stability, unfolding and substrate binding of RecA ☆
Leah Pandiscia, PhD
July 28, 2017
N5-carboxy-amino-imidazole ribonucleotide (N5-CAIR) mutase (PurE), a bacterial enzyme in the de novo purine biosynthetic pathway, has been suggested to be a target… Continue reading Identification of Bacillus anthracis PurE inhibitors with antimicrobial activity
Leah Pandiscia, PhD
July 28, 2017
Biophysical and structural characterization of G protein-coupled receptors (GPCRs) has been limited due to difficulties in expression, purification, and vitro stability of… Continue reading An expression and purification system for the biosynthesis of adenosine receptor peptides for biophysical and structural characterization
Leah Pandiscia, PhD
July 28, 2017
Leah Pandiscia, PhD
July 28, 2017
Leah Pandiscia, PhD
July 28, 2017
Thirteen previously undescribed 5,6,7,8-tetrahydro-2-(2-phenylethyl)chromones named tetrahydrochromone A–M, together with nine known ones, were isolated from artificial agarwood (induced by holing)… Continue reading 5,6,7,8-Tetrahydro-2-(2-phenylethyl)chromones from artificial agarwood of Aquilaria sinensis and their inhibitory activity against acetylcholinesterase
Leah Pandiscia, PhD
July 28, 2017
Increasing evidence is accumulating, showing that neurodegenerative disorders are somehow associated with the toxicity of amyloid aggregates, metal ion dyshomeostasis… Continue reading Multitarget trehalose-carnosine conjugates inhibit Aβ aggregation, tune copper(II) activity and decrease acrolein toxicity
Leah Pandiscia, PhD
July 28, 2017
This study focuses on the development and application of biophysical methodology to characterize conformations of Epogen® and Eprex®, the injectable… Continue reading Biophysical comparability of the same protein from different manufacturers: A case study using Epoetin alfa from Epogen® and Eprex®
Leah Pandiscia, PhD
July 28, 2017
Ricin is a potent toxin associated with bioterrorism for which no vaccine or specific countermeasures are currently available. A stable,… Continue reading Improved formulation of a recombinant ricin A-chain vaccine increases its stability and effective antigenicity
Leah Pandiscia, PhD
July 28, 2017
The effect of polyethyleneimine (PEI), a cationic polymer, on protein stability was investigated. Shelf stability of lactate dehydrogenase, stored as… Continue reading Protein stabilising effect of polyethyleneimine
Leah Pandiscia, PhD
July 28, 2017
Changes in protein–protein interactions, protein unfolding, and nonnative aggregation were assessed for a series of human IgG1 antibodies as a… Continue reading Comparative effects of pH and ionic strength on protein–protein interactions, unfolding, and aggregation for IgG1 antibodies
Leah Pandiscia, PhD
July 28, 2017
Advancements towards an improved vaccine against Bacillus anthracis, the causative agent of anthrax, have focused on formulations composed of the… Continue reading Amphiphilic Polyanhydride Nanoparticles Stabilize Bacillus anthracis Protective Antigen
Leah Pandiscia, PhD
July 28, 2017
We report the discovery of a DNA sequence that templates a highly stable fluorescent silver nanocluster. In contrast to other… Continue reading A DNA-templated fluorescent silver nanocluster with enhanced stability
Leah Pandiscia, PhD
July 28, 2017