Study of Protein Secondary Structure

Understanding Secondary Structure

Proteins adopt unique conformations, optimized to perform specific
functions. Therefore, the information on the protein structure is
paramount to elucidating how proteins function.

Sampling Methods for Secondary Structure Estimation

MethodFTIR SpectroscopyCircular Dichroism SpectroscopyX-Ray Crystallography
Sample FormSolid and Liquid
(Crystal and amorphous)
LiquidSolid (crystal)
Concentration5 - 100%0.25 - 2.5%100%
Temperature ControlYesYesYes
Auto TitrationNoYesNo
Secondary Structure
Under StudyWell-knownStandard Method
Information CollectedSSESSE, Tertiary StructureSSE, Tertiary Structure

Secondary Structure Estimation Using FTIR Spectroscopy

Infrared spectroscopy is an effective technique for component analysis and interaction analysis of biological samples such as proteins, sugars, and lipids. FTIR spectroscopy can be used to measure solid and liquid samples when applied to secondary structure analysis of proteins.

Secondary Structure Estimation Using Circular Dichroism