Title
Inhibition of HL-60 cell growth via cell cycle arrest and apoptosis induction by a cycloartane–labdane heterodimer from Pseudolarix amabilis
Author
Xinhui Tian, Niao Yang, Bo Li, Jianping Zhang, Xike Xu, Rongcai Yue, Huiliang Li, Liping Chen, Yunheng Shen, Weidong Zhang
Year
2016
Journal
Organic and Biomolecular Chemistry
Abstract
Pseudolaridimer C (1), a rarely encountered cycloartane–labdane Diels–Alder adduct was isolated from the cones of Pseudolarix amabilis. The structure and absolute configuration of 1 were established by comprehensive NMR and CD spectral analysis. The WST-8 assay indicated that 1time and dose dependently inhibited the proliferation of human leukemia cells HL-60 at 1–10 μM. DAPI and Annexin V-FITC/PI double staining method, and DNA ladder experiments all proved that1 had significant dose-dependent effects on HL-60 cell apoptosis. A further mechanism study indicated that the apoptosis was associated with the cell cycle arrest during the G2/M phase, and the activation of caspase-9, -3, -7, and poly-(ADP-ribose)-polymerase (PARP).
Instrument
J-810
Keywords
Circular dichroism, Absolute configuration, Organic chemistry, Biochemistry