Title
Interaction of fisetin with human serum albumin by fluorescence, circular dichroism spectroscopy and DFT calculations: binding parameters and conformational changes
Author
Ilulia Matei, Sorana Ionescu, Mihaela Hillebrand
Year
2011
Journal
Journal of Luminescence
Abstract
The interaction between fisetin, an antioxidant and neuroprotective flavonoid, and human serum albumin (HSA) is investigated by means of fluorescence (steady-state, synchronous, time-resolved) and circular dichroism (CD) spectroscopy. The formation of a 1:1 complex with a constant of about 105 M−1 was evidenced. Förster's resonance energy transfer and competitive binding with site markers warfarin and ibuprofen were considered and discussed. Changes in the CD band of HSA indicate a decrease in the α-helix content upon binding. An induced CD signal for bound fisetin was observed and rationalized in terms of density functional theory calculations.
Instrument
J-815
Keywords
Circular dichroism, Protein folding, Secondary structure, Biochemistry