Title
Enhanced protein retention on poly(caprolactone) via surface initiated polymerization of acrylamide
Author
Yuhao Maa, Mengtan Cai, Liu He, Xianglin Luo
Year
2016
Journal
Applied Surface Science
Abstract
To enhance the biocompatibility or extend the biomedical application of poly(caprolactone) (PCL), protein retention on PCL surface is often required. In this study, poly(acrylamide) (PAAm) brushes were grown from PCL surface via surface-initiated atom transfer radical polymerization (SI-ATRP) and served as a protein-capturing platform. Grafted PAAm was densely packed on surface and exhibited superior protein retention ability. Captured protein was found to be resistant to washing under detergent environment. Furthermore, protein structure after being captured was investigated by circular dichroism (CD) spectroscopy, and the CD spectra verified that secondary structure of captured proteins was maintained, indicating no denaturation of protein happened for retention process.
Instrument
J-1500
Keywords
Circular dichroism, Secondary structure, Polymers, Materials