A novel pyrimidine tetrad contributing to stabilize tetramolecular G-quadruplex structures

By V. Esposito, A. Pepe, R. Filosa, L. Mayol, A. Virgilio, A. Galeone

July 28, 2017

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Title

A novel pyrimidine tetrad contributing to stabilize tetramolecular G-quadruplex structures

Author

V. Esposito, A. Pepe, R. Filosa, L. Mayol, A. Virgilio, A. Galeone

Year

2016

Journal

Organic and Biomolecular Chemistry

Abstract

G-quadruplex structures formed by oligodeoxyribonucleotides TGGUNH2GGT (AM, UNH2 = 5-amino-2′-deoxyuridine), TGGUBrGGT (BR, UBr = 5-bromo-2′-deoxyuridine) and TGGTGGT (TH) have been investigated through circular dichroism, nuclear magnetic resonance, gel electrophoresis and molecular modeling techniques. Collected data indicate that all 7-mer oligonucleotides form tetramolecular parallel G-quadruplex structures with all residues adoptinganti glycosidic bonds. In the case of AM, data suggest the occurrence of a novel UNH2-tetrad characterized by eight hydrogen bonds that stabilizes the G-quadruplex structure more efficiently than UBr- and T-tetrads.

Full Article

http://pubs.rsc.org/en/content/articlelanding/2016/ob/c5ob02358k

Instrument

J-715

Keywords

Circular dichroism, Biochemistry