Title
Label Free Luminescence Strategy for Sensitive Detection of ATP using Aptamer-Ru(II) complexes
Author
Eththilu Babu, Paulpandian Muthu Mareeswaran, Arumugam Ramdass, Pandian Ramesh, Seenivasan Rajagopal
Year
2016
Journal
Journal of Luminescence
Abstract
A simple and sensitive aptamer-based luminescence strategy for ATP detection is developed using Ru(II) complexes as probe molecule. It is based on the fact that Ru(II)-dppz complexes show the light switching behavior with DNA aptamers and found to show significant luminescence spectral change on the addition of ATP molecules. The binding efficiencies of aptamer with ATP, ADP and AMP are calculated and compared. The structural change of aptamer is also studied using circular dichroism (CD) spectral techniques. Moreover, the binding nature of aptamer with ATP, ADP and AMP is demonstrated by computational techniques. The proposed strategy was successfully applied to the detection of ATP.
Instrument
J-810
Keywords
Circular dichroism, Ligand binding, Coordination chemistry