Title
Synthesis of bio-based aldehyde from seaweed polysaccharide and its interaction with bovine serum albumin
Author
Faisal Kholiya, Jai Prakash Chaudhary, Nilesh Vadodariya, Ramavatar Meena
Year
2016
Journal
Carbohydrate Polymers
Abstract
Here, we demonstrate a successful synthesis of bio-based aldehyde namely dialdehyde-carboxymethylagarose (DCMA) using carboxymethyagarose (CMA). Further reaction parameters (i.e. reaction temperature, pH and periodate concentration) were optimized to achieve maximum aldehyde content and product yield. The synthesis of DCMA was confirmed by employing FTIR, 1H NMR, XRD, SEM, AFM, TGA, DSC, EA and GPC techniques. To investigate the aldehyde functionality, DCMA was allowed to interact with BSA and obtained results were found to be comparable with that of synthetic aldehyde (Formaldehyde). Further interaction of DCMA with BSA was confirmed by using UV-Vis, FTIR, fluorescent spectroscopy, CD and DLS analysis. Results of this study revealed that bio-based aldehyde behaves like formaldehyde. This study adds value to abundant marine biopolymers and opens the new research area for polymer researchers.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Polymers, Ligand binding,