Title
Ligand-mediated G-quadruplex Induction in a Double-stranded DNA Context by Cyclic Imidazole/Lysine Polyamide
Author
Sefan Asamitsu, Yue Li, Toshikazu Bando, Hiroshi Sugiyama
Year
2016
Journal
A European Journal of Chemical Biology
Abstract
G-quadruplex (G4) DNA is often observed as a DNA secondary structure in guanine-rich sequences and is thought to be relevant to pharmacological and biological events. Therefore, G4 ligands have attracted great attention regarding potential anticancer therapies or molecular probe applications. In the present study, we designed cyclic imidazole/lysine polyamide (cIKP) as a new class of G4 ligand, which was readily synthesized without time-consuming column chromatography. cIKP enables the selective recognition of particular G4 structures with low nanomolar affinity. Moreover, cIKP exhibited an unprecedented property: the induction of G4 formation on the promoter of G4-containing DNAs in a stable double-stranded DNA (dsDNA) context under molecular crowding conditions. This cIKP may be applicable as a molecular probe for the detection of potential G4-forming sequences in dsDNA.
Instrument
J-805
Keywords
Circular dichroism, DNA structure, Ligand binding, Nanostructures, Biochemistry