Title
Visualization of G-quadruplexes in gel and in live cells by a near-infrared fluorescent probe
Author
Fan Wu, Chaoxing Liu, Yuqi Chen, Shixi Yang, Jiahui Xu, Rong Huang, Xiang Wang, Manjia Li, Wenting Liu, Wuxiang Mao, Xiang Zhou
Year
2016
Journal
Sensors and Actuators B: Chemical
Abstract
G-quadruplexes, one of the most significant secondary structure of nucleic acid, are formed by stacking G quartets, which received broad interests due to their involvement in telomere function, gene transcription and recombination. As for ligand, better selectivity for G-quadruplex against other DNA or RNA structures and higher ability to stabilize G-quadruplex are necessary. In addition, developing a new probe to recognize G-quadruplex is desired for utilizing G-quadruplex structure to relevant biological processes. In this study, we report a Cy5 labelled ligand (named PDP-Cy5) developed as a near-infrared fluorescent ligand for G-quadruplexes both in DNA and RNA. The results indicated that PDP-Cy5 selectively induced the formation of intramolecular G-quadruplexes with strong binding affinity. Furthermore, this Cy5 labelled ligand can effectively stabilize G-quadruplexes. Moreover, the direct visualization of G-quadruplexes in gel, even on cell level is realized by using PDP-Cy5.
Instrument
J-810
Keywords
Circular dichroism, DNA structure, Ligand binding, Materials, Biochemistry