Title
Inhibition of Fibrillar Assemblies of L-Phenylalanine by Crown Ethers: A Potential Approach towards Phenylketonuria
Author
Debasis Banik, Rupam Dutta, Pavel Banerjee, Sangita Kundu, and Nilmoni Sarkar Debasis Banik, Rupam Dutta, Pavel Banerjee, Sangita Kundu, Nilmoni Sarkar
Year
2016
Journal
The Journal of Physical Chemistry B
Abstract
In this article, our aim is to investigate the interaction of L-Phenylalanine (Phe) fibril with crown ethers (CEs). For this purpose, two different CEs (15-Crown-5 and 18-Crown-6) were used. Interestingly, we have observed that both CEs have the ability to arrest fibril formation. However, 18C6 was found to be a better candidate compared to 15C5. Field Emission Scanning Electron Microscopy (FESEM) and Fluorescence Lifetime Imaging Microscopy (FLIM) were used to monitor the fibril arresting kinetics by CEs. The arresting process was further confirmed by Fluorescence Correlation Spectroscopy (FCS) and Nuclear Magnetic Resonance (NMR) studies.
Full Article
Instrument
J-815
Keywords
Circular dichroism, Stereochemistry, Biochemistry