Structure Change from β-Strand and Turn to α-Helix in Histone H2A-H2B Induced by DNA Damage Response

By Yudai Izumi, Kentaro Fujii, Frank Wien, Chantal Houée-Lévin, Sandrine Lacombe, Daniela Salado-Leza, Erika Porcel, Rawand Masoud, Satoshi Yamamoto, Matthieu Réfrégiers, Marie-Anne Hervé du Penhoat, Akinari Yokoya

July 28, 2017

  • Year

  • Technique

  • Industry

Title

Structure Change from β-Strand and Turn to α-Helix in Histone H2A-H2B Induced by DNA Damage Response

Author

Yudai Izumi, Kentaro Fujii, Frank Wien, Chantal Houée-Lévin, Sandrine Lacombe, Daniela Salado-Leza, Erika Porcel, Rawand Masoud, Satoshi Yamamoto, Matthieu Réfrégiers, Marie-Anne Hervé du Penhoat, Akinari Yokoya

Year

2016

Journal

Biophysical Journal

Abstract

Using synchrotron radiation-based circular dichroism spectroscopy, we found that the DNA damage response induces an increase of α-helix structure and a decrease of β-strand and turn structures in histone H2A-H2B extracted from x-irradiated human HeLa cells. The structural alterations correspond to the assumption that an average of eight amino acid residues form new α-helix structures at 310 K. We propose the structural transition from β-strand and turn structures to an α-helix structure in H2A-H2B as a novel, to our knowledge, process involved in the DNA damage response.

Full Article

http://www.sciencedirect.com/science/article/pii/S000634951630399X

Instrument

J-725

Keywords

Circular dichroism, Secondary structure, Thermal stability, Biochemistry