Title
Structural and thermodynamic characterisation of L94F mutant of horse cytochrome c
Author
Sabab Hasan Khan, Atul kumar, Amresh Prakash, Bhupesh Taneja, Asimul Islam, Md. Imtiayaz Hassan, Faizan Ahmad
Year
2016
Journal
International Journal of Biological Macromolecules
Abstract
Mammalian mitochondrial cytochromes c (cyts c) has a conserved Leu94 which is replaced by valine/isoleucine in some lower eukaryotes and prokaryotes. It is expected that nature substituted Leu94 with Val/Ile, for they have similar van der Waals volume and hydrophobicity with a difference in side chain branching only. Reports also suggested the presence of phenylalanine at position 94, which leads to questions: (i) How bulky aromatic amino acid residue fitted at position 94 in cyt c family proteins? (ii) What is the effect of L94F mutation on protein stability and folding? Here, we selected horse cyt-c as a model to answer the second question. We generated L94F mutant of horse cytochromec and subsequently characterised using far-UV, near-UV and Soret circular dichroism, absorbance, intrinsic and extrinsic ANS (8-anilino-1-napthalenesulfonic acid) fluorescence and dynamic light scattering measurements. We observed that this mutation affects the native state and arrests the protein folding at the molten globule state. Thermal stability of L94F mutant is also measured by spectroscopic techniques and differential scanning calorimetry. The midpoint of thermal denaturation of L94F mutant is 17 °C less than wild type. Molecular dynamics simulation study also supports our in vitro observation that this mutant has stable backbone conformation.
Instrument
J-1500
Keywords
Circular dichroism, Secondary structure, Tertiary structure, Protein denaturation, Thermal stability, Thermodynamics, Biochemistry