Title
Nanocomposite based on modified TiO2–BSA for functional applications
Author
C.K. Simi, T. Emilia Abraham
Year
2009
Journal
Colloids and Surfaces B: Biointerfaces
Abstract
A novel nanocomposite based on TiO2–protein for functional applications was prepared and characterized. The composite was made by covalent immobilisation of bovine serum albumin (BSA) on to the TiO2 particles. BSA was attached to the TiO2 powder through a three-step process. This involves the functionalization of the TiO2 particles with silane and the subsequent coupling by glutaraldehyde to free NH2 groups of the protein. Formation of Schiff's base was confirmed by IR spectroscopy and the optimum loading of the BSA was found to be 73.61%. Surface morphology of the composite was studied by SEM and TEM. Thermal analysis of TiO2–BSA composite was carried out by TGA and DSC. Structural variation of the BSA after immobilisation was studied by CD spectra.
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Materials, Biochemistry