Title
Exploring the interaction of L-cysteine capped CuS nanoparticles with bovine serum albumin (BSA): a spectroscopic study
Author
S. Prasanth, D. Rithesh Raj, T. V. Vineeshkumar, Riju K. Thomas, C. Sudarsanakumar
Year
2016
Journal
RSC Advances
Abstract
The interactions between nanoparticles and proteins have prime importance in nanomedicine and nanotoxicology. L-Cysteine capped copper sulfide (Cy-CuS) nanoparticles having average particle size of 6 nm have been synthesized using chemical coprecipitation method and their interactions with Bovine Serum Albumin (BSA) were explored using various spectroscopic techniques. A ground state complex is formed between BSA and Cy-CuS nanoparticles in solution. The number of binding sites, binding constant, quenching constant and the binding distance between Cy-CuS nanoparticles and BSA were determined. The overall conformation of BSA in the complex was unaltered, however the α-helical content of BSA was marginally reduced.
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Nanostructures, Biochemistry, Materials