Title
Biophysical characterization of a model antibody drug conjugate
Author
Tsutomu Arakawa, Yasunori Kurosawa, Michael Storms, Toshiaki Maruyama, C. J. Okumura, Nasib Karl Maluf
Year
2016
Journal
Drug Discoveries & Therapeutics
Abstract
Antibody drug conjugates (ADC) are important next-generation biopharmaceuticals and thus require stringent structure characterization as is the case for monoclonal antibodies. We have tested several biophysical techniques, i.e., circular dichroism, analytical ultracentrifugation, differential scanning calorimetry and fluorescence spectroscopy, to characterize a fluorescein-labeled monoclonal antibody as a model ADC. These techniques indicated possible small structure and stability changes by the conjugation, while largely retaining the tertiary structure of the antibody, consistent with unaltered biological activities. Thus, the above biophysical techniques are effective at detecting changes in the structural properties of ADC.
Instrument
J-715
Keywords
Circular dichroism, Secondary structure, Tertiary structure, Antibodies, Biochemistry