Title
High-Throughput Screening of Identity, Enantiomeric Excess, and Concentration Using MLCT Transitions in CD Spectroscopy
Author
Sonia Nieto, Vincent M. Lynch, Eric V. Anslyn, Hyunwoo Kim, Jik Chin
Year
2008
Journal
JACS
Abstract
This manuscript describes a protocol for the fast determination of identity, enantiomeric excess (ee) and concentration of chiral 1,2-diamines, the combination of which has not previously been achieved, using the changes in the circular dichroism (CD) spectra of the charge-transfer bands of the Cu(I)-Binap complex. The spectra were analyzed with a variety of pattern recognition (PR) protocols. PR techniques were used to analyze unknown samples in a robotically controlled 96-well plate interfaced CD instrument. In less than two minutes per sample, ee and concentration values can be read with 2% and 8% error, respectively. The speed and accuracy as well as ability to simultaneously measure ee concentration and identity represent clear advantages over traditional methods and makes this method adaptable to true high-throughput screening.
Full Article
Instrument
J-815
Keywords
Circular dichroism, Coordination chemistry, Stereochemistry