Title
Evaluation of pendimethalin binding to human serum albumin: Insights from spectroscopic and molecular modeling approach
Author
Wei Qi Lee, Ida Syazwani M. Affandi, Shevin R. Feroz, Saharuddin B. Mohamad, Saad Tayyab
Year
2016
Journal
Journal of Biochemical and Molecular Toxicology
Abstract
Interaction of pendimethalin (PM) herbicide with the major transporter in human circulation, human serum albumin (HSA), was studied using fluorescence, circular dichroism (CD), and molecular modeling methods. The attenuation of the fluorescence intensity of HSA in the presence of PM revealed formation of the PM–HSA complex. Analysis of the fluorescence quenching data showed moderately strong binding affinity between PM and HSA. Both hydrophobic interactions and hydrogen bonding were suggested to stabilize the PM–HSA complex, based on thermodynamic data. Binding of PM to HSA induced perturbation in the microenvironment around the aromatic fluorophores as well as secondary and tertiary structural changes in the protein. Complexation of PM with HSA led to an increase in its thermal stability. Both site marker displacement and molecular modeling results suggested site I, located in subdomain IIA as the preferred binding site of PM on HSA.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Tertiary structure, Ligand binding, Biochemistry, Toxicology