Title
A biophysical investigation on the binding of proflavine with human hemoglobin: Insights from spectroscopy, thermodynamics and AFM studies
Author
Anirban Basu, Gopinatha Suresh Kumar
Year
2016
Journal
Journal of Photochemistry and Photobiology B: Biology
Abstract
Interaction of proflavine with hemoglobin (Hgb) was studied employing spectroscopy, calorimetry, and atomic force microscopy. The equilibrium constant was found to be of the order 104 M− 1. The quenching of Hgb fluorescence by proflavine was due to the complex formation. Calculation of the molecular distance (r) between the donor (β-Trp37 of Hgb) and acceptor (proflavine) suggested that energy can be efficiently transferred from the β-Trp37 residue at the α1β2 interface of the protein to the dye. Proflavine induced significant secondary structural changes in Hgb. Synchronous fluorescence studies showed that proflavine altered the microenvironment around the tryptophan residues to a greater extent than the tyrosine residues. Circular dichroism spectral studies showed that proflavine caused significant reduction in the α-helical content of Hgb. The esterase activity assay further complemented the circular dichroism data. The Soret band intensity of Hgb decreased upon complexation. Differential scanning calorimetry and circular dichroism melting results revealed that proflavine induced destabilization of Hgb. The binding was driven by large positive entropy contribution along with favorable negative enthalpy. Atomic force microscopy studies revealed that the essential morphological features of hemoglobin were retained in presence of proflavine. Overall, insights on the photophysical aspects and energetics of the binding of proflavine with Hgb are presented.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Thermal stability, Ligand binding, Thermodynamics, Biochemistry