Title
Effective construction of AuNPs-DNA system for the implementation of various advanced logic gates
Author
Chunyang Zhou, Changtong Wu, Yaqing Liu, Erkang Wang
Year
2016
Journal
RSC Advances
Abstract
In this work, we integrate DNA computing at the nanoscale on the basis of the self-assembly of single stranded polyA-DNA on the AuNPs as the precursor solution. As a “lab-on-a-nanoparticle”, the intelligent DNA strands modified AuNPs could be operated to realize a variety of Boolean logic gates including half adder, half subtractor, 2:1 encoder and 4:2 encoder in an enzyme-free condition for the first time. The signal readout methods, FAM fluorescence, G-quadruplex enhanced fluorescence of NMM and i-motif structure enhanced fluorescence of CV were used to report the final signal in different logic operations, respectively. The mechanisms were further investigated by native polyacrylamide gel electrophoresis. This investigations provide a wider field of vision towards prototypical DNA-based circuits in biological systems and promotes the development of advanced logic circuits.
Instrument
J-820
Keywords
Circular dichroism, DNA structure, Biochemistry