Title
Chemically modified cellulose strips with pyridoxal conjugated red fluorescent gold nanoclusters for nanomolar detection of mercuric ions
Author
Shilpa Bothra, Yachana Upadhyay, Rajender Kumar, S.K. Ashok Kumar, Suban K. Sahoo
Year
2016
Journal
Biosensors and Bioelectronics
Abstract
One-pot approach was adopted for the synthesis of highly luminescent near-infrared (NIR)-emitting gold nanoclusters (AuNCs) using bovine serum albumin (BSA) as a protecting agent. The vitamin B6 cofactor pyridoxal was conjugated with the luminescent BSA-AuNCs through the free amines of BSA and then employed for the nanomolar detection of Hg2+ in aqueous medium via selective fluorescence quenching of AuNCs. This nano-assembly was successfully applied for the real sample analysis of Hg2+ in fish, tap water and river water. The study also presents chemically-modified cellulosic paper strips with the pyridoxal conjugated BSA-AuNCs for detecting Hg2+ ion up to 1 nM.
Instrument
J-715
Keywords
Circular dichroism, Secondary structure, Ligand binding, Nanostructures, Biochemistry, Materials