Title
Reversible Vesicle-to-Disk Transitions of Liposomes Induced by Self-Assembly of Water-Soluble Porphyrins
Author
Kouta Sugikawa, Yutaro Takamatsu, Kazuma Yasuhara, Masafumi Ueda, Atsushi Ikeda
Year
2017
Journal
Langmuir
Abstract
Structural control of the self-assembled lipid membrane is an important and interesting challenge, both for revealing the mechanisms behind biological functions and for creating high-functionality soft materials. In this study, we developed a reversible structure control system of vesicles, also called liposomes, using supramolecular assemblies. Water-soluble anionic porphyrin molecules interacted with the surface of zwitterionic lipid membrane surfaces and formed one-dimensional self-assembled structures (J-aggregates) under acidic conditions. From cryo-TEM images, it was confirmed that the formation of porphyrin J-aggregates on the lipid membrane surface induced an extensive structural change in liposomes from vesicles to a layered-sheet structure. This structural change was reversible and the deformation of porphyrin J-aggregates, induced by neutralization of the solution, caused reconstruction of liposomes from layered-sheet structures.
Instrument
J-1500
Keywords
Circular dichroism, Vesicle interactions, Aggregation, Biochemistry