New Insights into Cooperative Binding of Homeodomain Transcription Factors PREP1 and PBX1 to DNA

By Chiara Zucchelli, Elena Ferrari, Francesco Blasi, Giovanna Musco, Chiara Bruckmann

July 28, 2017

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Title

New Insights into Cooperative Binding of Homeodomain Transcription Factors PREP1 and PBX1 to DNA

Author

Chiara Zucchelli, Elena Ferrari, Francesco Blasi, Giovanna Musco, Chiara Bruckmann

Year

2017

Journal

Scientific Reports

Abstract

PREP1 and PBX1 are homeodomain (HD) transcription factors that play crucial roles in embryonic development. Here, we present the first biophysical characterization of a PREP1 HD, and the NMR spectroscopic study of its DNA binding pocket. The data show that residues flanking the HD participate in DNA binding. The kinetic parameters for DNA binding of individual PREP1 and PBX1 HDs, and of their combination, show that isolated PREP1 and PBX1 HDs bind to DNA in a cooperative manner. A novel PREP1 motif, flanking the HD at the C-terminus, is required for cooperativity.

Full Article

http://www.nature.com/articles/srep40665

Instrument

J-815

Keywords

Circular dichroism, Secondary structure, Thermal stability, Thermodynamics, Biochemistry