Title
Eugenol prevents amyloid formation of proteins and inhibits amyloid-induced hemolysis
Author
Kriti Dubey, Bibin G. Anand, Dolat Singh Shekhawat, Karunakar Kar
Year
2017
Journal
Scientific Reports
Abstract
Eugenol has attracted considerable attention because of its potential for many pharmaceutical applications including anti-inflammatory, anti-tumorigenic and anti-oxidant properties. Here, we have investigated the effect of eugenol on amyloid formation of selected globular proteins. We find that both spontaneous and seed-induced aggregation processes of insulin and serum albumin (BSA) are significantly suppressed in the presence of eugenol. Isothermal titration calorimetric data predict a single binding site for eugenol-insulin complex confirming the affinity of eugenol for native soluble insulin species. We also find that eugenol suppresses amyloid-induced hemolysis. Our findings reveal the inherent ability of eugenol to stabilize native proteins and to delay the conversion of protein species of native conformation into β-sheet assembled mature fibrils, which seems to be crucial for its inhibitory effect.
Full Article
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Aggregation, Biochemistry