Title
Bacterial expression, correct membrane targeting and functional folding of the HIV-1 membrane protein Vpu using a periplasmic signal peptide
Author
Arpan Deb, William A. Johnson, Alexander P. Kline, Boston J. Scott, Lydia R. Meador, Dustin Srinivas, Jose M. Martin-Garcia, Katerina Dörner, Chad R. Borges, Rajeev Misra, Brenda G. Hogue, Petra Fromme, Tsafrir S. Mor
Year
2017
Journal
PLoS ONE
Abstract
Viral protein U (Vpu) is a type-III integral membrane protein encoded by Human Immunodeficiency Virus-1 (HIV- 1). It is expressed in infected host cells and plays several roles in viral progeny escape from infected cells, including down-regulation of CD4 receptors. But key structure/function questions remain regarding the mechanisms by which the Vpu protein contributes to HIV-1 pathogenesis. Here we describe expression of Vpu in bacteria, its purification and characterization. We report the successful expression of PelB-Vpu in Escherichia coli using the leader peptide pectate lyase B (PelB) from Erwinia carotovora. The protein was detergent extractable and could be isolated in a very pure form. We demonstrate that the PelB signal peptide successfully targets Vpu to the cell membranes and inserts it as a type I membrane protein. PelB-Vpu was biophysically characterized by circular dichroism and dynamic light scattering experiments and was shown to be an excellent candidate for elucidating structural models.
Instrument
J-710
Keywords
Circular dichroism, Secondary structure, Thermal stability, Ligand binding, Biochemistry