Title
An insight to the binding of Ellagic acid with Human serum albumin using spectroscopic and isothermal calorimetry studies
Author
Rudradip Pattanayak, Pijush Basak, Srikanta Sen, Maitree Bhattacharyya
Year
2017
Journal
Biochemistry and Biophysics Reports
Abstract
Ellagic acid (EA), a natural polyphenol evidence several pharmacological benefits. The binding profile of EA with human serum albumin (HSA) has been explored and investigated by Isothermal titration calorimetry (ITC), circulardichroism (CD) spectroscopy, time-correlated single-photon counting (TCSPC), absorbance spectroscopy, steady-state fluorescence spectroscopy, and modelling studies. The ITC data analysis revealed the binding Constant (Ka), ΔH, ΔS and ΔG values to be 15.5 × 104M-1, −116.2±18.1 Kcal mol-1, −366 cal mol-1K-1 and −7.13 Kcal mol-1respectively with a unique binding site at HSA. EA effectively quenched the intrinsic fluorescence of HSA by static quenching, whereas TCSPC data also revealed association of dynamic quenching also. Thermodynamic analysis confirmed that hydrophobic and mainly hydrogen bonding interaction played important role in stabilizing the HSA-EA complex. It further dictates the binding reaction to be enthalpy driven. The secondary structure of HSA was altered upon binding with EA. CD spectroscopic data indicated the fraction of alpha helicity to be decreased from 52% to 40% upon binding to EA. This study will provide an insight on evaluation of this bioactive interaction during transport and releasing efficiency at the target site in human physiological system since HSA is the most important carrier protein in blood serum.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Ligand binding, Biochemistry