Title
Comprehensive insight into the binding of sunitinib, a multi-targeted anticancer drug to human serum albumin
Author
Md. Zahirul Kabir, Wei-Ven Tee, Saharuddin B. Mohamad, Zazali Alias, Saad Tayyab
Year
2017
Journal
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Abstract
Binding studies between a multi-targeted anticancer drug, sunitinib (SU) and human serum albumin (HSA) were made using fluorescence, UV-vis absorption, circular dichroism (CD) and molecular docking analysis. Both fluorescence quenching data and UV-vis absorption results suggested formation of SU–HSA complex. Moderate binding affinity between SU and HSA was evident from the value of the binding constant (3.04 × 104 M−1), obtained at 298 K. Involvement of hydrophobic interactions and hydrogen bonds as the leading intermolecular forces in the formation of SU–HSA complex was predicted from the thermodynamic data of the binding reaction. These results were in good agreement with the molecular docking analysis. Microenvironmental perturbations around Tyr and Trp residues as well as secondary and tertiary structural changes in HSA upon SU binding were evident from the three-dimensional fluorescence and circular dichroism results. SU binding to HSA also improved the thermal stability of the protein. Competitive displacement results and molecular docking analysis revealed the binding locus of SU to HSA in subdomain IIA (Sudlow's site I). The influence of a few common ions on the binding constant of SU–HSA complex was also noticed.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Tertiary structure, Ligand binding, Biochemistry