Title
Monitoring thermal and chemical unfolding of Brugia malayi calreticulin using fluorescence and Circular Dichroism spectroscopy
Author
Sunita Yadav, Smita Gupta, Jitendra Kumar Saxena
Year
2017
Journal
International Journal of Biological Macromolecules
Abstract
Calreticulin of Brugia malayi (BmCRT) play very important role in host-parasite interaction. In previous study it was found that BmCRT is responsible for prevention of host classical complement pathway activation via its interaction with first component C1q of the human host. Therefore, BmCRT is an essential protein for parasite survival and an important drug target to fend filariasis. In the present study, we have carried out a systamatic biophysical characterization of BmCRT protein. Unfolding of BmCRT was found to be non-cooperative two-state process in the presence of both denaturant GdmCl and urea. The results also illustrated that protein lost its 50% activity at 1.5 M GdmCl and 3 M Urea. Partially unfolded and molten-globule like intermediate state was observed at 0.8 to 1.2 M GdmCl while Urea unfolding showed intermediate state at 1.2 to 1.6 M. Unfolding pathway monitored with the help of apolar quencher, favor above observations. All of these findings support the presence of detectable intermediate state during unfolding pathway of BmCRT. Furthermore, this study indicates that BmCRT is more stable towards temperature (Tm = 65 °C), pH and trypsin digestion. These differences in properties as compared to host can be fruitfully utilized for synthesis of compounds effective against the parasite.
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Protein denaturation, Thermal stability, Chemical stability, Thermodynamics, Biochemistry