Title
The Dimerization State of the Mammalian High Mobility Group Protein AT-Hook 2 (HMGA2)
Author
Lorraine Frost, Maria A. M. Baez, Christopher Harrilal, Alyssa Garabedian, Francisco Fernandez-Lima, Fenfei Leng
Year
2015
Journal
PLoS ONE
Abstract
The mammalian high mobility group protein AT-hook 2 (HMGA2) is a chromosomal architectural transcription factor involved in cell transformation and oncogenesis. It consists of three positively charged “AT-hooks” and a negatively charged C-terminus. Sequence analyses, circular dichroism experiments, and gel-filtration studies showed that HMGA2, in the native state, does not have a defined secondary or tertiary structure. Surprisingly, using combined approaches of 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) chemical cross-linking, analytical ultracentrifugation, fluorescence resonance energy transfer (FRET), and mass spectrometry, we discovered that HMGA2 is capable of self-associating into homodimers in aqueous buffer solution. Our results showed that electrostatic interactions between the positively charged “AT-hooks” and the negatively charged C-terminus greatly contribute to the homodimer formation.
Instrument
J-720
Keywords
Circular dichroism, Secondary structure, Biochemistry