Title
Synthesis, characterization, computational study, and biological relevance of a family of isostructural, mononuclear Ln (Ln = Gd, Tb, Dy, Ho, Er) complexes containing pyridoxine, an essential ingredient of vitamin B6 enzyme
Author
Caitlyn E. Stouder, Khairi J. Warren, Olivia F. Perdue, Amanda L. Stewart, Clifford W. Padgett, Allison J. Amonette, Arpita Saha
Year
2017
Journal
Inorganica Chimica Acta
Abstract
The synthesis and characterization of a family of LnIIIcomplexes (Ln = Gd(1), Tb(2), Dy(3), Ho(4) and Er (5)) of formula [(LnIII(NO3)2(OH2)(pyridoxine)2](NO3)are reported, where pyridoxine is 2-methyl-3-hydroxy-4,5-bis(hydroxymethyl)pyridine. They were obtained from the reaction of Ln(NO3)3with pyridoxine in ethanol in a 1:1 molar ratio. The crystal structures of representative 1 and 5 were obtained using a single-crystal X-ray diffractometer. The subsequent characterization of all the complexes was performed using elemental analysis, IR spectroscopy and UV-Vis spectroscopy. The gadolinium complex was further analyzed to determine if DNA is a biological target of this series of metal complexes. In this regard, the interaction between the gadolinium complex and DNA was probed utilizing circular dichroism spectroscopy. The study supports the moderate binding of complex 1 to DNA.
Instrument
J-815
Keywords
Circular dichroism, DNA structure, Ligand binding, Biochemistry, Inorganic chemistry