Remote Measurement using FP-8500 Fluorometer with Optical Fiber
January 5, 2024
Introduction
![FP-8500 Spectrofluorometer](https://jascoinc.com/wp-content/uploads/2017/05/FP8500PC.png)
A fluorometer with optical fiber can be used for a variety of measurements, such as when the sample is larger than the sample chamber, to follow an in-situ reaction, or for measurement in hazardous environments, such as high/low temperature and high pressure. The OBF-832 optical fiber interface is used to connect an optical fiber for remote sampling of fluorescence materials with an FP-8000 Series fluorescence spectrophotometer. By using an optical fiber, measurement can be made by holding the probe close to or immersed in the sample.
In this application note, a comparison was made by measuring samples using an FP-8500 fluorometer with optical fiber and an FLH-809 film holder. To check for spectral accuracy the fiber probe was corrected for quantum efficiency using a calibrated light source with a validated spectral emission.
Experimental
Measurement System and Condition
FP-8500 | Fluorescence spectrophotometer |
OBF-832 | Optical fiber unit |
Calibration | ESC-842 Calibration light source / standard white reference plate |
Samples | Red and Yellow reference cards |
System Configuration
Measurement of the Calibrated Light Source under Dark Room Condition
![Measurement Condition](https://jascoinc.com/wp-content/uploads/2017/08/APP-Note-FP-0018-fig-1-b.jpg)
Measurement Condition
Measurement Mode | Fluorescence |
Excitation Bandwidth | 10 nm |
Emission Bandwidth | 10 nm |
Excitation Wavelength | 310 nm |
Measurement Range | 400 - 800 nm |
Scan Speed | 100 nm/min |
Data Interval | 0.5 nm |
Response | 1 sec |
PMT Voltage | 350 V (yellow card), 550 V (red card) |
Keywords
260-FP-0018, Spectrofluorometer, Optical fiber, Fluorescence spectrum, fluorescence spectrophotometer
Results
Figures 2 and 3 show the measurement results. The profiles of the spectra of each colored sample measured using both the optical fiber and FLH-809 film holder were consistent with each other.
These results demonstrate that the spectral accuracy obtained using both the fiber probe and film holder yield comparable data.
![Normalized fluorescence spectra of the yellow card](https://jascoinc.com/wp-content/uploads/2017/08/APP-Note-FP-0018-fig-2.jpg)
![Normalized fluorescence spectra of the red card](https://jascoinc.com/wp-content/uploads/2017/08/APP-Note-FP-0018-fig-3.jpg)
Required Products and Software
- FP-8300/8500/8600/8700 Spectrofluorometer
- OBF-832 Optical Fiber Unit
- ESC-842 Calibrated WI Light Source
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![JASCO Application Note](https://jascoinc.com/wp-content/uploads/2023/10/app-pdf-header-2.png)
Remote Measurement using FP-8500 Fluorometer with Optical Fiber
Introduction
![FP-8500 Spectrofluorometer](https://jascoinc.com/wp-content/uploads/2017/05/FP8500PC.png)
A fluorometer with optical fiber can be used for a variety of measurements, such as when the sample is larger than the sample chamber, to follow an in-situ reaction, or for measurement in hazardous environments, such as high/low temperature and high pressure. The OBF-832 optical fiber interface is used to connect an optical fiber for remote sampling of fluorescence materials with an FP-8000 Series fluorescence spectrophotometer. By using an optical fiber, measurement can be made by holding the probe close to or immersed in the sample.
In this application note, a comparison was made by measuring samples using an FP-8500 fluorometer with optical fiber and an FLH-809 film holder. To check for spectral accuracy the fiber probe was corrected for quantum efficiency using a calibrated light source with a validated spectral emission.
Experimental
Measurement System and Condition
FP-8500 | Fluorescence spectrophotometer |
OBF-832 | Optical fiber unit |
Calibration | ESC-842 Calibration light source / standard white reference plate |
Samples | Red and Yellow reference cards |
System Configuration
Measurement of the Calibrated Light Source under Dark Room Condition
![Measurement Condition](https://jascoinc.com/wp-content/uploads/2017/08/APP-Note-FP-0018-fig-1-b.jpg)
Measurement Condition
Measurement Mode | Fluorescence |
Excitation Bandwidth | 10 nm |
Emission Bandwidth | 10 nm |
Excitation Wavelength | 310 nm |
Measurement Range | 400 - 800 nm |
Scan Speed | 100 nm/min |
Data Interval | 0.5 nm |
Response | 1 sec |
PMT Voltage | 350 V (yellow card), 550 V (red card) |
Keywords
260-FP-0018, Spectrofluorometer, Optical fiber, Fluorescence spectrum, fluorescence spectrophotometer
Results
Figures 2 and 3 show the measurement results. The profiles of the spectra of each colored sample measured using both the optical fiber and FLH-809 film holder were consistent with each other.
These results demonstrate that the spectral accuracy obtained using both the fiber probe and film holder yield comparable data.
![Normalized fluorescence spectra of the yellow card](https://jascoinc.com/wp-content/uploads/2017/08/APP-Note-FP-0018-fig-2.jpg)
![Normalized fluorescence spectra of the red card](https://jascoinc.com/wp-content/uploads/2017/08/APP-Note-FP-0018-fig-3.jpg)
Required Products and Software
- FP-8300/8500/8600/8700 Spectrofluorometer
- OBF-832 Optical Fiber Unit
- ESC-842 Calibrated WI Light Source