Title
Cisplatin Binding Sites in Human H-Chain Ferritin
Author
Giarita Ferraro, Silvia Ciambellotti, Luigi Messori, Antonello Merlino
Year
2017
Journal
Inorganic Chemistry
Abstract
The aim of this work is to identify the cisplatin binding sites on human H-chain ferritin. High-resolution X-ray crystallography reveals that cisplatin binds four distinct protein sites, that is, the side chains of His136 and Lys68, the side chain of His105, the side chain of Cys90 and the side chain of Cys102. These Pt binding sites are compared with those observed for the adduct that cisplatin forms upon encapsulation within horse spleen L-chain ferritin (87% identity with human L-chain ferritin).
Instrument
J-710
Keywords
Circular dichroism, Secondary structure, Ligand binding, Thermal stability, Inorganic chemistry, Biochemistry