Title
An activity transition from NADH dehydrogenase to NADH oxidase during protein denaturation
Author
Scott Huston, John Collins, Fangfang Sun, Ting Zhang, Timothy D. Vaden, Y.-H. Percival Zhang, Jinglin Fu
Year
2017
Journal
Biotechnology and Applied Biochemistry
Abstract
A decrease in the specific activity of an enzyme is commonly observed when the enzyme is inappropriately handled or is stored over an extended period. Here, we reported a functional transition of an FMN-bound diaphorase (FMN–DI) that happened during the long-term storage process. It was found that FMN–DI did not simply lose its β-nicotinamide adenine diphosphate (NADH) dehydrogenase activity after a long-time storage, but obtained a new enzyme activity of NADH oxidase. Further mechanistic studies suggested that the alteration of the binding strength of an FMN cofactor with a DI protein could be responsible for this functional switch of the enzyme.
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Protein folding, Soret, Ligand binding, Biochemistry