Title
The interaction of Schiff Base complexes of nickel(II) and zinc(II) with duplex and G-quadruplex DNA
Author
Riccardo Bonsignore, Fabrizia Russo, Alessio Terenzi, Angelo Spinello, Antonino Lauria, Giuseppe Gennaro, Anna Maria Almerico, Bernhard K. Keppler, Giampaolo Barone
Year
2018
Journal
Journal of Inorganic Biochemistry
Abstract
The duplex and G-quadruplex DNA-binding of six nickel(II) and zinc(II) complexes of three salphen-like ligands (salphen = N,N′-bis-salicylidene-1,2-phenylenediaminato) was investigated by UV–visible absorption and circular dichroism spectroscopy. The results obtained, in particular the values of the DNA-binding constants, Kb, point out that the nickel(II) complexes show a higher affinity toward both duplex and G-quadruplex DNA, compared to the analogous zinc(II) complexes. Interestingly, the zinc(II) complexes possess high selectivity toward G-quadruplex DNA, being negligible their binding with duplex DNA. Molecular dynamics (MD) simulations provided atomistic models for the interpretation of the binding of the zinc(II) complexes with G-quadruplex DNA, allowing a structural comparison of the three salphen-like ligands, due to the presence of different substituents (H, F, CF3) on the position 4 of the phenyl ring on the N,N′ bridge.
Instrument
J-715
Keywords
Circular dichroism, DNA structure, Thermal stability, Thermodynamics, Ligand binding, Inorganic chemistry, Biochemistry