Title
A General Microwave Synthesis of Metal (Ni, Cu, Zn) Selenide Nanoparticles and Their Competitive Interaction with Human Serum Albumin
Author
Sambandam Anandan, Naveenraj Selvaraj, Mangalaraja R Viswanathan, Olga Krasulyaa, Asad Syed, Fuad Ameen
Year
2018
Journal
New Journal of Chemistry
Abstract
A series of selenide nanoparticles (3 1 nm sized platelet-like NiSe nanoparticles, uniform CuSe nanorods with ca. 12 nm width and 65 nm in length, and distorted ZnSe nano-hexagons with side length 12 3.5 nm) have been synthesized by simple microwave irradiation technique using sodium selenite, hydrazine hydrate and starch as selenide precursor, reducing agent and stabilizing agent, respectively. The morphologies and sizes of the as-synthesized nanoparticles were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM) and energy-dispersive X-ray spectroscopy (EDS) analysis. The interaction between this series of selenide nanoparticles (SNPs) and HSA was investigated using fluorescence and circular dichroism (CD) spectroscopy. The influencing factors such as the quenching type, binding stoichiometries, binding constants, and the free energy change determined from the fluorescence technique showed that SNPs spontaneously bound to HSA in the ratio of 1:1 through non-fluorescent ground-state complex formation (static quenching mechanism). The binding constant values indicated that the binding forces were in a descending order of NiSe > CuSe > ZnSe. The shift in the synchronous fluorescence spectra signified the involvement of the tryptophan moiety in the binding of SNPs with HSA. Based on the Förster theory of energy transfer, the distance between the donor (Trp residues) and the acceptor (SNPs) was obtained. Analysis of the far-UV and near-UV CD spectra of HSA suggested the effect of the SNPs on the secondary and tertiary structure of HSA. These investigations helped us to understand the interaction mechanisms between the nanoparticles and the protein molecule that interprets the pharmacokinetics of these nanoparticles while administering it as drugs.
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Nanostructures, Tertiary structure, Biochemistry, Materials