Title
Recognition motif and mechanism of ripening inhibitory peptides in plant hormone receptor ETR1
Author
Dalibor Milić, Markus Dick, Daniel Mulnaes, Christopher Pfleger, Anna Kinnen, Holger Gohlke, Georg Groth
Year
2018
Journal
Scientific Reports
Abstract
Synthetic peptides derived from ethylene-insensitive protein 2 (EIN2), a central regulator of ethylene signalling, were recently shown to delay fruit ripening by interrupting protein–protein interactions in the ethylene signalling pathway. Here, we show that the inhibitory peptide NOP-1 binds to the GAF domain of ETR1 – the prototype of the plant ethylene receptor family. Site-directed mutagenesis and computational studies reveal the peptide interaction site and a plausible molecular mechanism for the ripening inhibition.
Full Article
Instrument
J-715
Keywords
Circular dichroism, Secondary structure, Biochemistry