Title
Covalent modification of soy protein isolate by (–)‐epigallocatechin‐3‐gallate: effects on structural and emulsifying properties
Author
Fei Tao, He Jiang, Wenwei Chen, Yongyong Zhang, Jiarong Pan, Jiaxin Jiang, Zhenbao Jia
Year
2018
Journal
Journal of the Science of Food and Agriculture
Abstract
Soy protein isolate (SPI) has promising applications in various food products because of its excellent functional properties and nutritional quality. The structural and emulsifying properties of covalently modified SPI by (–)‐epigallocatechin‐3‐gallate (EGCG) were investigated. SPI was covalently modified by EGCG under alkaline conditions. SDS‐PAGE analysis revealed that EGCG modification caused cross‐linking of SPI proteins. Circular dichroism spectra demonstrated that the secondary structure of SPI proteins was changed by EGCG modification. In addition, the modifications resulted in the perturbation of the tertiary structure of SPI as evidenced by intrinsic fluorescence spectra and surface hydrophobicity measurements. Oil‐in‐water emulsions of modified SPI had smaller droplet sizes and better creaming stability compared to those from unmodified SPI. The covalent modification by EGCG improved the emulsifying property of SPI. This study provided an innovative approach for improving the emulsifying properties of proteins.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Biochemistry, Food science