Title
Fluorescent-labeled single-strand ATP aptamer DNA: Chemo- and enantio-selectivity in sensing adenosine
Author
Hidehito Urata, Kanba Nomura, Shun-ichi Wada, Masao Akagi
Year
2007
Journal
Biochemical and Biophysical Research Communications
Abstract
One of the intriguing applications of aptamers is sensing molecules. In principle, an aptamer can specifically recognize and bind to a unique ligand, leading to a structural change of an aptamer. By acquiring information for the structural change, the detection of the ligand can be achieved. To design and explore an aptamer molecule to detect adenosine, we have synthesized some ATP aptamer variants labeled with donor and acceptor fluorophores. Although the fluorescent response of the aptamer variants was highly dependent on experimental temperature, we have found one of the variants showing suitable fluorescent response by titration with adenosine. The aptamer variant showed remarkable selectivity for adenosine over the other ribonucleosides. On the other hand, the enantio-specificity of the aptamer variant in the ligand recognition was not enough to selectively detect d-adenosine over l-adenosine.
Instrument
FP-6500
Keywords
Fluorescence, FRET, DNA structure, Thermal stability, Ligand binding, Biochemistry, Sensors