Title
A comparative study: Metal nanoparticles as fluorescent sensors for biomolecules and their biomedical application
Author
Bharat A. Makwana, Savan Darjee, Vinod K. Jain, Anita Kongor, Gaurang Sindhav, Mandava V. Rao
Year
2017
Journal
Sensors and Actuators B: Chemical
Abstract
In the present study, the interaction of the calix[4]resorcinarene polyhydrazide (CPH) capped nanoparticles (CPH-AuNps) and (CPH-AgNps) with different amino acids like arginine, aspartic acid, glutamic acid, glycine, leucine, methionine, histidine, tryptophan and cysteine has been studied by using UV–vis and fluorescence spectroscopy. CPH-AuNps and CPH-AgNps were found to be the selective and sensitive fluorescent probe for l-DOPA and histidine, respectively. The minimum detectable limit for both l-DOPA and histidine was found at 10 nM by a facile way of fluorescence quenching i.e. by a turn off mechanism. CPH-AuNps and CPH-AgNps have also been studied for their antioxidant activity and interaction with DNA by UV–vis spectrophotometry. Furthermore, electrophoresis technique confirmed the interaction of ethidium bromide (EtBr) intercalated CT-DNA and S-DNA with CPH-AuNps and CPH-AgNps, respectively. The simplicity, sensitivity and specificity for the detection of amino acids by the CPH-AuNps and CPH-AgNps, indicates that the synthesized nanoparticles can be used as nano biosensor, with a potential prospect in the biomedical analysis.
Instrument
FP-6500
Keywords
Fluorescence, Nanostructures, Biochemistry, Materials, Sensors