Title
Electrophoretic Deposition (EPD) of Lectin in the Presence of New Glycopolymers Aiming at Facile Detection of Carbohydrate–Protein Interactions
Author
Tomomi Komura, Akinori Takasu
Year
2017
Journal
Macromolecular Chemistry and Physics
Abstract
Atom transfer radical polymerization of allyl methacrylate followed by a thiol-ene click reaction with 1-thio-β-D-glucose tetraacetate is performed to afford a new type of glycopolymers via deacetylation. Their interactions with a sugar-binding protein are evaluated by electrophoretic deposition (EPD) procedures as well as fluorescence measurements. The deposition amount of concanavalin A (conA) in the presence of glucose or galactose measured by EPD is remarkably different from that in the presence of the synthesized glycopolymer, even though the concentration of sugar residue is the same. The deposition amount, as well as the electric current that flows during the EPD, is higher in the presence of the polymer, which confirms that interaction of conA with the glycopolymer is detected by the EPD procedure.
Instrument
FP-8500
Keywords
Fluorescence, Protein structure, Ligand binding, Biochemistry