Title
Multilayered controlled drug release silk fibroin nano-film by manipulating secondary structure
Author
Moonhyun Choi, Daheui Choi, Jinkee Hong
Year
2018
Journal
Biomacromolecules
Abstract
Many studies of drug delivery nano-platforms have explored drug loading affinity or controlled release. The nano-platforms can be influenced by their inherent building blocks. Natural polypeptide silk fibroin (SF) is an excellent nano-platform material because of its high biocompatibility and unique structural properties. SF secondary structures have different properties that can be changed by external stimuli. Thus, the characterization of SF-containing platforms is strongly affected by secondary structure transformations. Structural changes can occur spontaneously, which hinders the control of structural variation in aqueous conditions. Herein, we successfully prepared a controllable secondary structure composed of SF/heparin (HEP) layer-by-layer assembled nanofilms using simple solvents (glycerol and methanol). SF in the SF/HEP nanofilms takes up than 90%, which means configurations of SF have a strong effect on the characters of the nanofilms. We investigated the degradation profiles of SF/HEP nanofilms depending on their -sheet contents and demonstrated an immediate correlation between the transformation of secondary structures inside the nanofilms and the degradation rate of nanofilms. Finally, SF/HEP nanofilms were used as a delivery platform for incorporating the anti-cancer drug epirubicin (EPI). We could control the loading efficiency and release profile of EPF with various -sheet contents of the nanofilms.
Instrument
FP-8300
Keywords
Fluorescence, Ligand binding, Kinetics, Nanostructures, Chemical stability, Medicinal, Biochemistry, Materials