Title
On/Off Fluorescent Chemosensor for Selective Detection of Divalent Iron and Copper Ions: Molecular Logic Operation and Protein Binding
Author
G. Tamil Selvan, Chitra Varadaraju, R. Tamil Selvan, Israel V. M. V. Enoch, P. Mosae Selvakumar
Year
2018
Journal
ACS Omega
Abstract
Here, naphthalene diamine-based β-diketone derivative (compound LH) was successfully used as a dual signaling probe for divalent cations, Fe2+ and Cu2+ ions, in bimodal methods (colorimetric and fluorometric). It showed fluorescent enhancement for Fe2+ ion by photoinduced electron transfer mechanism and fluorescence quenching for Cu2+ ion by charge-transfer process. Binding stoichiometry for [LH–(Fe2+)2] and [LH–(Cu2+)2] was found to be 1:2 by Job’s plot method and, the binding constants were calculated as 1.6638 × 1010 and 9.22929 × 108 M–1, respectively. Compound LH exhibited OR and XOR logic gate behavior with H+, Fe2+, and Cu2+ as inputs. Further, the compound LH and bovine serum albumin binding interaction showed quenching of fluorescence by Förster resonance energy-transfer mechanism.
Instrument
FP-8300
Keywords
Fluorescence, Ligand binding, Sensors, Chemical stability, FRET, Quenching, Optical properties, Materials