Title
Interplay between CedA, rpoB and double stranded DNA: A step towards understanding CedA mediated cell division in E. coli
Author
Pankaj Sharma, Anil Kumar Tomar, Bishwajit Kundu
Year
2018
Journal
International Journal of Biological Macromolecules
Abstract
Cell division is compromised in DnaAcos mutant E. coli cells due to chromosome over-replication. In these cells, CedA acts as a regulatory protein and initiates cell division by a hitherto unknown mechanism. CedA, a double stranded DNA binding protein, interacts with various subunits of RNA polymerase complex, including rpoB. To reveal how this concert between CedA, rpoB and DNA brings about cell division in E. coli, we performed biophysical and in silico analysis and obtained mechanistic insights. Interaction between CedA and rpoB was shown by circular dichroism spectrometry and in silico docking experiments. Further, CedA and rpoB were allowed to interact individually to a selected DNA and their binding was monitored by fluorescence spectroscopy. The binding constants of these interactions as determined by BioLayer Interferometry clearly show that rpoB binds to DNA with higher affinity (KD2 = < 1.0E–12 M) as compared to CedA (KD2 = 9.58E–09 M). These findings were supported by docking analysis where 12 intermolecular H-bonds were formed in rpoB-DNA complex as compared to 4 in CedA-DNA complex. Based on our data we propose that in E. coli cells chromosome over-replication signals CedA to recruit rpoB to specific DNA site(s), which initiates transcription of cell division regulatory elements.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Chemical stability, Biochemistry