Title
Colorimetric and fluorescence signalling of thioesculetin in presence of oxidising agent
Author
Rupali G Shinde, Ayesha A Khan, Atanu Barik
Year
2018
Journal
Journal of Chemical Sciences
Abstract
Thioesculetin (TE) is a sulfur analogue of esculetin. The UV-Vis absorption maximum of TE at 470 nm shifted to 357 nm in the presence of oxidising agent such as m-chloroperoxy benzoic acid (m-CPBA). With gradual increase in the m-CPBA concentration, the absorption band at 470 nm decreases while the band at 357 nm increases. The same system shows gradual increase in the fluorescence signal at 463 nm in the presence of m-CPBA. The fluorescence signal was unaffected in the presence of common anions and metal cations. The mechanism for optical signalling was due to the conversion of weakly fluorescent TE to fluorescent esculetin through desulfurization reaction.
Instrument
FP-8500
Keywords
Fluorescence, Optical properties, Chemical stability, Sensors, Kinetics, Materials