Title
Sequential backbone resonance assignment of AT-rich interaction domain of human BAF200
Author
Abhishek Bastiray, Malyasree Giri, Mahavir Singh
Year
2018
Journal
Biomolecular NMR Assignments
Abstract
BAF200 is a subunit of PBAF chromatin remodeling complex that contains an N-terminal AT-rich interaction domain (ARID). ARID domain in general has been shown to bind to the AT-rich DNA sequences. The human BAF200 ARID (~ 110 residues) has the potential to bind the DNA sequences with high affinity, however, the structure and the exact contribution of hBAF200 ARID in PBAF functions as well its DNA binding specificities have not been established. In this study, we have expressed and purified the hBAF200 ARID for NMR studies. We report the complete backbone 1H, 13C, and 15N chemical shift assignment and secondary structure of hBAF200 ARID domain.
Instrument
J-715
Keywords
Circular dichroism, Secondary structure, Biochemistry